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Current Research on Biosciences and Biotechnology
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26861623     DOI : https://doi.org/10.5614/crbb
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology
The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a multidisciplinary environment. We encourage scientists to submit papers focusing on the following broad topics: Health and Medicine Pharmaceutical Sciences Pharmacology and Toxicology Food Microbiology and Biotechnology Biotechnology Agriculture Bio-Energy Marine Sciences Advanced Materials Environmental Sciences
Arjuna Subject : Umum - Umum
Articles 6 Documents
 1 
Search results for , issue "Vol. 4 No. 1 (2022)" : 6 Documents clear
Total phenolic, flavonoid, and antioxidant capacity of bajakah (Spatholobus littoralis Hassk) Muhammad Ikhlas Arsul; Nurfajri Syamsi; Nadila Putri; Nur Afiah Alfrianti Nur; Mukhriani; Nursalam Hamzah
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/VRJ3X4LF

Abstract

The bajakah (Spatholobus littoralis Hassk.) is one of the typical plants from Kalimantan Island, which has potential as a drug, one of which using as an antioxidant is due to the secondary metabolites contained therein. The aims of the research are to estimate the total phenolic content, total flavonoid content, and antioxidant capacity of the bajakah by different methods. Extraction was carried out by reflux method using a solvent gradient of n-hexane, ethyl acetate, and ethanol 96%. Phenols and flavonoid content were calculated using spectroscopy. Antioxidant capacity was measured against ascorbic acid and Trolox using the CUPRAC, DPPH, and FRAP methods and reported as mg Trolox equivalent (TEAC) and ascorbic acid equivalent (AEAC) per gram extract. The ethanol extract showed the highest antioxidant capacity for the CUPRAC and FRAP methods, the n-hexane extract for the DPPH method on both varieties of bajakah tampala. Each method provides significantly different antioxidant capacity values. The CUPRAC and FRAP methods found the highest antioxidant capacity in the white variety of bajakah ethanol extract. Meanwhile, in the DPPH method, saw the highest antioxidant capacity in the red variety of n-hexane bajakah extract. DPPH and CUPRAC give a positive correlation with TPC, where CUPRAC assay produced higher values than FRAP and DPPH assays.
Isolation, identification and biochemical studies of gallic acid from Turbinaria decurrens Bory Fitriyanti Jumaetri Sami; Syamsu Nur
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/X7DD09RY

Abstract

Cancer is one of the diseases that cause the biggest death in the world. The research for natural compounds derived from marine is very developed at this time, especially in algae. Brown algae have more phenolic content than other types of algae, T. decurrens Bory is one of the brown algae that had taken from the Indonesian island of Dutungan, South Sulawesi. Phenolic groups are also known to be able to inhibit the growth of cancer cells. The purpose of this study is to isolate and identify compounds using spectrophotometer instruments. The isolates obtained were tested for anticancer activity against H460 and MCF-7 cells. Extraction was carried out by maceration method, fractionation was done by chromatography column eluent n-hexane:EtOAc: MeOH by increasing polarity. The structure determination is done based on the interpretation of FT-IR, 1D-NMR 1H,13C, and ESI-LCMS spectra data and anticancer activity test used MTT bioassay. The isolate is known as gallic acid, and the isolate was tested for anticancer activity against H460 and MCF-7 cells. The result is obtained IC50 value for H460 cells 5.69 μg/ml and MCF-7 cells 4.63 μg/ml. As a positive control used cisplatin with an IC50 value of 5.81 μg/ml against H460 cells and MCF-7 cells with a value of 5.59 μg/ml. Gallic acid has a higher toxic effect compared to cisplatin. One phenolic compound has been found in T. decurrens Bory which is gallic acid. Gallic acid has a higher toxic effect than cisplatin, this compound can be used as an anticancer agent.
Lipid content and fatty acid profile of commonly consumed freshwater and marine fish of Bangladesh Mohammad Shoeb; Md. Mazharul Islam; Khandoker Tahmina Tasnim; Sunjida Akter; Md. Iqbal Rouf Mamun
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/J1LQ0XTP

Abstract

The objectives of this research were to determine total lipid content and corresponding fatty acid profiles in freshwater (n=10) and marine fish (n=11) samples commonly consumed in Bangladesh. Lipid was extracted by Solid Phase Dispersion method. Saponification and esterification were carried out by the association of official analytical chemists (AOAC) reference procedure with some simple modifications. The fatty acids were analyzed as their methyl ester by gas chromatography equipped with flame ionization detector (GC-FID) by comparing the retention time of 13 standard methyl ester of fatty acids. Total lipid content was 0.97-8.05% and 0.97-4.33% in freshwater and marine fish, respectively. Unsaturated fatty acid in freshwater fish was found in highest amount than saturated fatty acid. Among unsaturated fatty acids, palmitoleic acid was found in highest concentration and ranged from 46.80-26.02%. Palmitic acid was found in highest amount among saturated fatty acids and ranged from 3.96-13.91%. Among the marine fishes unsaturated fatty acids (MUFAs and PUFAs) i.e; palmitoleic acid (26.21-41.61%), oleic acid (2.35-29.63%) and linoleic acid (1.40-14.45%) were predominant. Among the saturated fatty acids myristic acid (0.97-9.08%), palmitic acid (1.61-11.35%) and stearic acid (1.03-21.53%) were found to be predominant.
Antihypertensive activity and acute toxicity of turmeric (Curcuma longa L.) in L-NAME-induced hypertension animals Patonah Hasimun; Yani Mulyani; Irma Yulianti
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/B2AMBRI1

Abstract

Hypertension is one of the significant risk factors for cardiovascular disease and can lead to complications. Uncontrolled hypertension can lead to vascular endothelial dysfunction and other complications of cardiovascular disease. This study aimed to determine the effect of turmeric rhizome (Curcuma longa L.) in extracts and the fractions on a hypertensive rat’s model induced by L-NAME 40 mg/kg for three weeks and an acute toxicity study of the extract. Antihypertensive research was performed on male Wistar rats utilizing non-invasive procedures. Turmeric extract at doses of 50, 100, and 200 mg/kg and its fraction of n-hexane, acetyl acetate, and ethanol at a dose of 25 mg/kg, respectively, were given daily per oral for three weeks to 2.5 mg/kg captopril. The systolic and diastolic blood pressure were measured at 0, 21, and 42 days after treatment and was calculated as mean arterial blood pressure (MAP). Acute toxicity testing refers to the OECD 420 Fixed-Dose method with several dosage levels, consists of 300, 2000, and 5000 mg/kg. The turmeric extract at doses of 50, 100, and 200 mg/kg and its fraction of n-hexane, acetyl acetate, and ethanol significantly reduced mean arterial blood pressure (p<0.05) compared to the control group. Acute administration of turmeric extracts up to a dose of 5000 mg/kg in test animals did not show any death. Turmeric and its components are considered to possess antihypertensive actions. Antihypertensive activity increased in a dose-dependent manner. Turmeric extract is categorized as being almost entirely non-toxic.
Biological activity studies of the aerial parts of Phyllanthus niruri L. Md. Nazrul Islam; Mohammad Shoeb; Nilufar Nahar
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/U7GMG56E

Abstract

Phyllanthus niruri L. is a small annual plant grows up to 30-40 cm tall. It is an herbaceous plant that is widely distributed in the tropical and sub-tropical regions. The aerial parts of P. niruri growing in Bangladesh were investigated for moisture content and ash content, total phenolic content, total flavonoid content, total antioxidant capacity (TAC), cytotoxicity, relative fatty acid compositions and antibacterial activity. The moisture and ash content of the dried leaves were 5 and 7%, respectively. The extracts of P. niruri contained a good amount of phenolic and flavonoid content and revealed significant antioxidant capacity. The highest TAC was shown by dichloromethane fraction (131.3±9.20) and it was followed by 80% ethanol extract (126.1±6.25) and then ethyl acetate fraction (122.5±10.65). The highest amount of phenolic content was present in ethyl acetate fraction, whereas the lowest amount was in hexane fraction. Cytotoxicity was found in 80% ethanol extract, dichloromethane and aqueous fractions. Fatty acids like lauric acid (0.5%), tetradecanoic acid (1%) palmitoleic acid (37%),octadecanoic acid (57%) cis-9-oleic acid (5%) were identified and quantified in hexane fraction of P. niruri by GC-FID and octadecanoic acid was predominant among all acids. The antibacterial activity of 80% ethanol extract and aqueous fraction of P. niruri was tested against two Gram negative (Escherichia coli, Salmonella typhi) and two Gram positive (Bacillus subtilis and Staphylococcus aureus) bacteria following agar diffusion method. The results revealed weak antibacterial activity.
Method development of quercitrin enrichment from asthma-plant (Euphorbia hirta L.) using aromatic macroporous resin Sumail Sidik Ode Ishak; Amrianto; Diah Astari Salam; Rika Hartati
Current Research on Bioscences and Biotechnology Vol. 4 No. 1 (2022)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2022.4.1/2G0E50F6

Abstract

Asthma-plant contains high amount of quercitrin which make it a potential new source for flavonoids. This study aims to develop a method of quercitrin enrichment by utilizing macroporous resin, which is known to be safer, more eco-friendly, economics, and efficient. Evaluations were conducted over the performance and separation characteristics of the macroporous resin in quercitrin enrichment as well as the adsorption and desorption of quercitrin by the macroporous resin. The results showed that the adsorption process of the macroporous resin in relation to the amount of quercitrin in the extract were in accordance with the second order model, which means that the process of adsorption is affected by other compounds. Furthermore, the examination of the isotherm adsorption fit the Freundlich’s model (R2 = 0.9850) rather than the Langmuir’s one (R2 = 0.4334). In the optimal condition, the enrichment of quercitrin by using macroporous resin increased the abundance of quercitrin by nearly five times, from 3.60% of quercitrin content in the extract to 17.02% in the quercitrin-rich fraction, with recovery yield of 50.39%.

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